Enzyme lab

 The enzyme lab was really fun. It took up three full days and it was really easy so my group messed around while we waited. For the lab we had to take a computer over to the lab station and hook it up to a pressure sensor on logger pro. While one of us was doing that another person in the group was busy making the water yeast solution.  On the first day we used 3 ml water and 3 ml H2O2 in a test tube. Then we added x amount of drops and put the pressure gauge on top and recorded it on the computer. The graph below is from the first day. We put 15 drops then 20 drops then 45 drops. As you can see the graph peaked at 20 drops. I don't know what happened with the 45 drops.

The Second day we kept the number of drops the same and changed the Ph level. We used 15 drops of yeast and changed the Ph level to Ph 4 Ph 7 and Ph 10. As you can see below the 4 and 10 were the same and the Ph 7 was lower. Ph 4 is acidic so it should have killed the enzyme. It would have in a human but the enzyme in the yeast was able to beat the acid so it could break down the H2O2. The graph below is the Ph graph.

The last day we kept the drops the same again and changed the temperature. Again we used 15 drops of yeast. We changed the temperature to 0, 25, 38, and 80 degrees Celsius. The graph peaked at 38 degrees. which is right above body temperature. Above 38 degrees it is too hot for the enzymes to work as well. You can see this in the graph below.